Antibodies A Laboratory Manual Free Download



Antibodies: A Laboratory Manual, second edition

Edward A. Greenfield, editor

Description It has been 25 years since the now classic lab manual Antibodies, by Harlow and Lane appeared. This second edition of Antibodies, edited by Edward A. Greenfield of the Dana-Farber Cancer Institute builds on the core strengths of the first edition, presenting clear and authoritative protocols with extensive background information and troubleshooting advice.

Cold Spring Harbor Laboratory Press

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Twenty-five years after its original publication, Antibodies: A Laboratory Manual has now been revised and updated in a second edition, reflecting advances in understanding of the immune system and evolving methods of studying it. Chapters include 'Immunizing Animals', 'Antibody Purification and Storage', 'Immunoblotting', 'Cell Staining', 'Electrophoresis', 'Protein Techniques', and much more. Charts and some black-and-white photographs enhance this highly technical, in-depth, comprehensive reference and resource for advanced students, instructors, and professionals in the field, Antibodies: A Laboratory Manual is an absolute 'must-have' for hospital and college library medical bookshelves.

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Title Annotation:The Biology Shelf; Antibodies: A Laboratory Manual, 2nd ed.
Publication:Internet Bookwatch
Article Type:Book review
Date:Dec 1, 2013
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Description

It has been 25 years since the now classic lab manual Antibodies, by Harlow and Lane appeared. This second edition of Antibodies, edited by Edward A. Greenfield of the Dana-Farber Cancer Institute builds on the core strengths of the first edition, presenting clear and authoritative protocols with extensive background information and troubleshooting advice. The original introductory chapters have been recast and updated to take into account our current understanding of the immune system. The critical chapters on generating monoclonal antibodies and growing hybridomas, which demystified hybridoma generation, have been greatly expanded and updated to make these procedures easy to follow and adaptable to current research needs. The remaining chapters contain all new protocols and topics and reflect the progress in how antibodies are studied and used since the appearance of the original edition. These include:
  • 001dantibody purification and storage
  • 001dengineering antibodies, including use of degenerate oligonucleotides, 5'-RACE, phage display, and mutagenesis
  • 001dextensive labeling techniques
  • 001dnew immunoblotting protocols
  • 001dthe latest screening and labeling techniques
As Dr. Greenfield notes in his preface to this second edition: 'The Antibodies manual provided our laboratory with guidance in the form of protocols and recommendations for setting up a hybridoma facility. Everything we needed to know to make a monoclonal antibody was all there, neatly packaged in an easy-to-understand book..the second edition is intended to provide the necessary information and protocols to assist investigators with their first monoclonal antibody effort as well as to provide guidance for more experiences antibody makers who are having some difficulties with a particular project.'

What's New in This Edition?

I have the first edition. Why should I buy the second edition?

While the core of the first edition appears in updated and recast form in the second edition, more than half of the second edition contains entirely new information and protocols to reflect the changes in the field since the first edition.

Antibodies A Laboratory Manual Free Download Version

To whom is this edition addressed? Is this a book for immunologists?

Antibodies A Laboratory Manual Free Download

As with the first edition, the second edition provides information and protocols for both the neophyte and the experienced investigator. It dissects the mysteries of producing the antibodies and reagents you need for your experiments with the needs of molecular biologists in mind. It is the perfect book for new members of your lab as it has sufficient background information to provide context as well as clear, step-by-step instructions for performing the needed experiments—both the why and the how for these techniques. And if things go wrong, there is extensive troubleshooting to diagnose the problem.

Are the protocols in this book better than those available for download on the Web? Rfid handbuch finkenzeller pdf readeryalasopa.

What Is A Antibodies Test

Many of the protocols available on the Web are perfectly good, but there are many that are not. Reagents are expensive and deadlines are tight. CSHL Press manuals provide protocols that have been formulated and tested in the labs of leading investigators in the field. They are reliable and they work. And they include the context and troubleshooting information that many online protocols do not have.